![]() Ship chilled, except for ParaPak® which should be kept at room temperature. Intestinal swab or fecal swab in anaerobic transport media shipped at room temperatureĬlostridium perfringens genotyping PCR to further identify which genes for which toxins are presentįresh feces in leak-proof sterile containerįresh fecal sample in sterile container (fill to line on container but do not overfill) or in ParaPak® transport media to assist with Salmonella recovery Tied off loop of bowel refrigerated and less than 24 hour old or frozen If history indicates may also want to consider Brucella ovis ELISA* which is performed on serum. If convalescent samples will not be collected, or when infectious abortion is strongly suspected due to history, immediate testing of acute sample may be warranted. ![]() To avoid testing acute samples twice, hold and submit with convalescent samples. If second, convalescent samples are later submitted, tests on both samples will be run in parallel and fees for both samples will be charged. If acute samples are submitted alone please indicated if all tests should be performed. When acute and convalescent samples are/will be collected, for most serology assays they should always be tested in parallel, and paired results interpreted. Maternal heparin whole blood (green top tube) Maternal EDTA whole blood (lavender top tube) If indicated, add Virus Isolation (VI) on Fresh tissue: lung, placenta including cotyledons, abomasal contents, kidney, liver, heart, adrenal, skin. If indicated, add Border Disease SN (OBDVSN) to heart blood (serum), peritoneal, pleural or pericardial fluid in sterile red top tube. If placentitis present, consider adding Coxiella burnetii PCR (COXPCR) to placenta if not available use abomasal contents if neither available use lung. Ship fresh tissue chilled and protect fixed tissue from freezing. If full necropsy desired, see necropsy testing. Submit 2 fresh tissue samples: placenta including cotyledons and fetal kidney stomach contents acceptableįresh tissue: liver placenta including cotyledons, adrenal, intestine, spleen, kidney, lung Placenta including cotyledons if no placenta use lungįetal heart blood or pleural, pericardial or peritoneal effusion in a red top tubeįormalin fixed tissue: placenta, liver, lung, brain, adrenal, heart, thymus, small intestine, kidney, fetal skin Liver preferred placenta if liver unavailable ![]() If either one is not available, use abomasal contents in a red top tube. Submit 2 fresh tissue samples: placenta including cotyledons and liver. Submit 3 fresh tissue samples: placenta, lung, and stomach contents - labelled and individually bagged for individual aerobic cultureįresh lung if not available, use spleen or heartīovine Virus Diarrhea Virus (BVDV) SN, Type 1įetal heart blood or pleural, pericardial or peritoneal effusion in a red top tube.īovine Virus Diarrhea Virus (BVDV) SN, Type 2 See Small Animal Internal Medicine, 5th Edition, 2014, Nelson RW and Couto CG Chapter 88: Fever of Unknown Origin, pages 1279-1282 for other ideas of testing. This plan is intended to be used as an adjunct to targeted, disease-specific testing appropriate to the species, clinical history and geographic location or conditions. Viral causes of illness are best detected acutely and most are unlikely to be detected in a patient with a chronic fever of unknown origin. This diagnostic plan is designed to detect a broad spectrum of systemic bacterial, viral, and some blood parasite causes of acute fever of unknown origin, as well as certain inflammatory changes which may assist in determining the underlying problems. It may be helpful to collect and bank a serum sample for possible acute and convalescent serology. To order blood culture bottles, go to shipping supplies or call 607.253.3935. ![]() See Blood Culture Technique for more information. Ship chilled except for blood culture bottles and slides which are shipped at room temperature. Nasal swab in 0.5ml sterile saline to keep moist (not wet) Heparinized plasma (separated from a green top tube) or (2) tubes EDTA whole blood (lavender top tube) (3) inoculated anaerobic blood culture bottles (3) inoculated aerobic/fungal blood culture bottles To diagnose septicemia post-mortem fresh lung, kidney, spleen, and lymph node (tracheobronchial preferred) If not previously performed a large animal chemistry panel may be warranted and serum (separated) or heparinized plasma would need to be submitted. If case is chronic, virus isolation can be omitted if desired.
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